2NX3
Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.381, 198.528, 125.834 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 29.790 - 2.100 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.800 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.069 |
| Number of reflections | 206008 |
| <I/σ(I)> | 6.9 |
| Completeness [%] | 0.9 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
