2K31

Solution Structure of cGMP-binding GAF domain of Phosphodiesterase 5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phosphodiesterase 5A, cGMP-specific	polymer	176	19968.5	1	UniProt (Q0VBW0) Pfam (PF01590) UniProt (by SIFTS) (Q8CG03)	Mus musculus (mouse)
2	B (A)	GUANOSINE-3',5'-MONOPHOSPHATE	non-polymer		345.2	1	Chemie (35G)

Sequence modifications

A: 154 - 320 (UniProt: Q0VBW0)

PDB	External Database	Details
Met 153	-	initiating methionine
Glu 295	Ala 295	engineered mutation
Glu 302	Ile 302	engineered mutation
Leu 321	-	expression tag
Glu 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	19968.5
Non-Polymers*	Number of molecules	1
	Total formula weight	345.2
All*	Total formula weight	20313.7

*Water molecules are not included.