2J9F

Human branched-chain alpha-ketoacid dehydrogenase-decarboxylase E1b

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	2-OXOISOVALERATE DEHYDROGENASE ALPHA SUBUNIT	polymer	400	45581.1	2	UniProt (P12694) Pfam (PF00676)	HOMO SAPIENS (HUMAN)	BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE E1 COMPONENT ALPHA CHAIN, BCKDH E1-ALPHA, BCKDE1A
2	B, D (B, D)	2-OXOISOVALERATE DEHYDROGENASE BETA SUBUNIT	polymer	350	38845.2	2	UniProt (P21953) Pfam (PF02779) Pfam (PF02780)	HOMO SAPIENS (HUMAN)	BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE E1 COMPONENT BETA CHAIN, BCKDH E1-BETA
3	E, J (A, C)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	F, H, K, M (A, B, C, D)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
5	G, L (A, C)	C2-1-HYDROXY-3-METHYL-PROPYL-THIAMIN DIPHOSPHATE	non-polymer		496.4	2	Chemie (THV)
6	I, N, O (B, D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	P, Q, R, S (A, B, C, D)	water	water		18.0	1069	Chemie (HOH)

Sequence modifications

A, C: 1 - 400 (UniProt: P12694)

PDB	External Database	Details
Pro 302	Ser 347	engineered mutation

B, D: 1 - 342 (UniProt: P21953)

PDB	External Database	Details
Gly 343	-	expression tag
Gly 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	168852.8
Non-Polymers*	Number of molecules	11
	Total formula weight	1535.4
All*	Total formula weight	170388.1

*Water molecules are not included.