2IN8

crystal structure of Mtu recA intein, splicing domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endonuclease PI-MtuI	polymer	139	15307.4	1	UniProt (P0A5U4) Pfam (PF14890) UniProt (by SIFTS) (P9WHJ3) In PDB	Mycobacterium tuberculosis
2	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3		water	water		18.0	114	Chemie (HOH)

Sequence modifications

A: 1 - 102 (UniProt: P0A5U4)

PDB	External Database	Details
Gly 24	Asp 275	engineered mutation
Leu 67	Val 318	engineered mutation
Val 95	Gln 346	engineered mutation
Arg 96	Pro 347	engineered mutation
Asp 97	Arg 348	engineered mutation
Val 98	Arg 349	engineered mutation
Glu 99	Phe 350	engineered mutation
Thr 100	Asp 351	engineered mutation
Glu 102	Phe 353	engineered mutation

A: 404 - 440 (UniProt: P0A5U4)

PDB	External Database	Details
Gly 422	Asp 673	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	15307.4
Non-Polymers*	Number of molecules	1
	Total formula weight	96.1
All*	Total formula weight	15403.5

*Water molecules are not included.