2IN8
crystal structure of Mtu recA intein, splicing domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-01-01 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.870, 49.270, 64.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.520 - 1.700 |
R-factor | 0.252 |
Rwork | 0.252 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2IMZ |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.068 | 0.354 |
Number of reflections | 13488 | |
<I/σ(I)> | 8.9 | 3.1 |
Completeness [%] | 98.6 | 92.3 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 65% ammonium sulfate 100 mM Tris.HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |