2IIE

single chain Integration Host Factor protein (scIHF2) in complex with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Phage P H' site	polymer	35	10802.0	1		synthetic construct
2	D	DNA (5'-D(*DGP*DGP*DCP*DCP*DAP*DAP*DAP*DAP*DAP*DAP*DGP*DCP*DAP*DTP*DT)-3')	polymer	15	4611.0	1		synthetic construct
3	E	DNA (5'-D(*DGP*DCP*DTP*DTP*DAP*DTP*DCP*DAP*DAP*DTP*DTP*DTP*DGP*DTP*DTP*DGP*DCP*DAP*DCP*DC)-3')	polymer	20	6074.9	1		synthetic construct
4	A	Integration host factor	polymer	204	22884.9	1	UniProt (P0A6X7) UniProt (P0A6Y1) Pfam (PF00216) In PDB	Escherichia coli	IHF-alpha, IHF-beta
5	C, D, E, A	MANGANESE (II) ION	non-polymer		54.9	7	Chemie (MN)
6		water	water		18.0	275	Chemie (HOH)

Sequence modifications

A: 47 - 138 (UniProt: P0A6X7)
A: 4 - 41 (UniProt: P0A6Y1)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
Ser 3	-	expression tag

A: 141 - 195 (UniProt: P0A6Y1)

PDB	External Database	Details
Gly 42	-	linker
Gly 43	-	linker
Ser 44	-	linker
Gly 45	-	linker
Gly 46	-	linker
Gly 139	-	linker
Gly 140	-	linker
Gly 196	-	expression tag
Ser 197	-	expression tag
Gly 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	44372.8
Non-Polymers*	Number of molecules	7
	Total formula weight	384.6
All*	Total formula weight	44757.4

*Water molecules are not included.