2IIE
single chain Integration Host Factor protein (scIHF2) in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.371, 55.311, 177.833 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.410 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ihf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.110 |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.540 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Number of reflections | 18596 | |
| Completeness [%] | 99.0 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1:1 mix water/ well solution containing:50mM Tris (pH 7.5), 15% PEG5000-MME, 10% glycerol, 50mM NaCl, 30mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG5000-MME | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | sodium chloride | ||
| 5 | 1 | 1 | Manganese dichloride | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG5000-MME | ||
| 8 | 1 | 2 | sodium chloride | ||
| 9 | 1 | 2 | Manganese dichloride |
