2I3Q

Q44V mutant of Homing Endonuclease I-CreI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*AP*CP*GP*TP*GP*AP*GP*TP*CP*AP*GP*TP*TP*TP*CP*G)-3'	polymer	24	7418.8	1
2	D	5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*AP*CP*TP*CP*AP*CP*GP*TP*CP*GP*TP*TP*TP*TP*GP*C)-3'	polymer	24	7320.7	1
3	A, B	DNA endonuclease I-CreI	polymer	153	17560.2	2	UniProt (P05725) Pfam (PF00961) In PDB	Chlamydomonas reinhardtii	23S rRNA intron protein
4	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5		water	water		18.0	147	Chemie (HOH)

Sequence modifications

A, B: 1 - 153 (UniProt: P05725)

PDB	External Database	Details
Ala 28	Lys 28	engineered mutation
Thr 42	Ala 42	engineered mutation
Val 44	Gln 44	engineered mutation
Glu 110	Trp 110	engineered mutation
Gln 111	Arg 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49860.0
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	49940.1

*Water molecules are not included.