2HZM

Structure of the Mediator head subcomplex Med18/20

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	RNA polymerase II mediator complex subunit 20	polymer	212	23076.2	4	UniProt (P34162) Pfam (PF08612)	Saccharomyces cerevisiae (baker's yeast)	Suppressor of RNA polymerase B 2, Hyper-recombination suppressor protein 2
2	B, D, F, H (B, D, F, H)	RNA polymerase II mediator complex subunit 18	polymer	317	35497.8	4	UniProt (P32585) Pfam (PF09637)	Saccharomyces cerevisiae (baker's yeast)	Suppressor of RNA polymerase B 5
3	I (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	J, K, L, M, N... (A, B, C, D, E...)	water	water		18.0	229	Chemie (HOH)

Sequence modifications

A, C, E, G: 1 - 210 (UniProt: P34162)

PDB	External Database	Details
Ala -1	-	cloning artifact
Ser 0	-	cloning artifact

B, D, F, H: 2 - 307 (UniProt: P32585)

PDB	External Database	Details
Val 274	Ala 274	engineered mutation
Ala 308	-	cloning artifact
Ala 309	-	cloning artifact
Ala 310	-	cloning artifact
Leu 311	-	cloning artifact
Glu 312	-	cloning artifact
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	234296.0
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	234390.9

*Water molecules are not included.