2HE0

Crystal structure of a human Notch1 ankyrin domain mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Notch1 preproprotein variant	polymer	253	27634.7	2	UniProt (P46531) Pfam (PF00023) Pfam (PF12796)	Homo sapiens (human)	Human Notch ankyrin domain mutant
2	C (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
3	D, E (A, B)	water	water		18.0	416	Chemie (HOH)

Sequence modifications

A, B: 1 - 243 (UniProt: P46531)

PDB	External Database	Details
Ala 58	Glu 1930	engineered mutation
Ala 66	Arg 1938	engineered mutation
Leu 244	-	cloning artifact
Glu 245	-	cloning artifact
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55269.4
Non-Polymers*	Number of molecules	1
	Total formula weight	62.1
All*	Total formula weight	55331.4

*Water molecules are not included.