2H99

Crystal structure of the effector binding domain of a BenM variant (R156H,T157S)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	HTH-type transcriptional regulator benM	polymer	312	35612.0	2	UniProt (O68014) Pfam (PF00126) Pfam (PF03466) In PDB	Acinetobacter sp.	Ben and cat operon transcriptional regulator
2	A, B	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
3	A, B	ACETATE ION	non-polymer		59.0	5	Chemie (ACT)
4	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	578	Chemie (HOH)

Sequence modifications

A, B: 1 - 304 (UniProt: O68014)

PDB	External Database	Details
His 156	Arg 156	engineered mutation
Ser 157	Thr 157	engineered mutation
Leu 305	-	expression tag
Glu 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	71223.9
Non-Polymers*	Number of molecules	12
	Total formula weight	903.1
All*	Total formula weight	72127.0

*Water molecules are not included.