2GWC

Crystal structure of plant glutamate cysteine ligase in complex with a transition state analogue

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Glutamate cysteine ligase	polymer	449	51818.3	8	UniProt (O23736) Pfam (PF04107)	Brassica juncea	Gamma-glutamylcysteine synthetase, Gamma-ECS, GCS
2	I, K, M, O, Q... (A, B, C, D, E...)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
3	J, L, N, P, R... (A, B, C, D, E...)	(2S)-2-amino-4-(S-butylsulfonimidoyl)butanoic acid	non-polymer		222.3	8	Chemie (BSC)
4	AA, BA, CA, DA, EA... (C, D, E, F, G...)	water	water		18.0	1017	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 66 - 514 (UniProt: O23736)

PDB	External Database	Details
Mse 119	Met 119	modified residue
Mse 141	Met 141	modified residue
Mse 195	Met 195	modified residue
Mse 202	Met 202	modified residue
Mse 216	Met 216	modified residue
Mse 224	Met 224	modified residue
Mse 228	Met 228	modified residue
Mse 238	Met 238	modified residue
Mse 239	Met 239	modified residue
Mse 257	Met 257	modified residue
Mse 291	Met 291	modified residue
Mse 306	Met 306	modified residue
Mse 329	Met 329	modified residue
Mse 344	Met 344	modified residue
Mse 384	Met 384	modified residue
Mse 386	Met 386	modified residue
Mse 431	Met 431	modified residue
Mse 496	Met 496	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	414546.2
Non-Polymers*	Number of molecules	16
	Total formula weight	1972.9
All*	Total formula weight	416519.1

*Water molecules are not included.