2GWC
Crystal structure of plant glutamate cysteine ligase in complex with a transition state analogue
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9797, 0.9778, 0,.9737 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.040, 198.920, 114.970 |
Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
Resolution | 99.500 - 2.180 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.24126 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.479 |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.500 | 2.270 |
High resolution limit [Å] | 2.180 | 2.180 |
Number of reflections | 197271 | |
<I/σ(I)> | 13.6 | 2.3 |
Completeness [%] | 95.5 | 89.9 |
Redundancy | 4.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% PEG 3350, 0.2 M magnesium acetate tetrahydrate, 0.1 M tricine, 0.01 M L-buthionine(S,R)sulfoximine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |