2GWC
Crystal structure of plant glutamate cysteine ligase in complex with a transition state analogue
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797, 0.9778, 0,.9737 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.040, 198.920, 114.970 |
| Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
| Resolution | 99.500 - 2.180 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24126 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.479 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.500 | 2.270 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 197271 | |
| <I/σ(I)> | 13.6 | 2.3 |
| Completeness [%] | 95.5 | 89.9 |
| Redundancy | 4.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% PEG 3350, 0.2 M magnesium acetate tetrahydrate, 0.1 M tricine, 0.01 M L-buthionine(S,R)sulfoximine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
