2GT4
Crystal Structure of the Y103F mutant of the GDP-mannose mannosyl hydrolase in complex with GDP-mannose and MG+2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | GDP-mannose mannosyl hydrolase | polymer | 160 | 18408.6 | 3 | UniProt (P32056) Pfam (PF00293) | Escherichia coli K12 | GDPMH, Colanic acid biosynthesis protein wcaH |
| 2 | D, G, J (A, B, C) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 3 | E, H, K (A, B, C) | GUANOSINE-5'-DIPHOSPHATE-ALPHA-D-MANNOSE | non-polymer | 605.3 | 3 | Chemie (GDD) | |||
| 4 | F, I (B, C) | beta-D-mannopyranose | non-polymer | 180.2 | 2 | Chemie (BMA) | |||
| 5 | L, M, N (A, B, C) | water | water | 18.0 | 345 | Chemie (HOH) |
Sequence modifications
A, B, C: 2 - 160 (UniProt: P32056)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Phe 103 | Tyr 102 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 55225.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2249.3 | |
| All* | Total formula weight | 57475.1 |
