2GGV

Crystal structure of the West Nile virus NS2B-NS3 protease, His51Ala mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	non-structural protein 2B	polymer	56	5981.4	1	UniProt (Q203W3) UniProt (by SIFTS) (P06935)	West Nile virus	NS2B
2	B (B)	non-structural protein 3	polymer	185	20035.6	1	UniProt (Q203W3) UniProt (by SIFTS) (P06935)	West Nile virus	NS3
3	C, D (A, B)	water	water		18.0	90	Chemie (HOH)

Sequence modifications

A: 49 - 93 (UniProt: Q203W3)

PDB	External Database	Details
Met 48	-	cloning artifact
Gly 94	-	SEE REMARK 999
Gly 95	-	SEE REMARK 999
Gly 96	-	SEE REMARK 999
Gly 97	-	SEE REMARK 999
Ser 98	-	SEE REMARK 999
Gly 99	-	SEE REMARK 999
Gly 100	-	SEE REMARK 999
Gly 101	-	SEE REMARK 999
Arg 102	-	SEE REMARK 999
Arg 103	-	SEE REMARK 999

B: 2 - 178 (UniProt: Q203W3)

PDB	External Database	Details
Ala 51	His 1556	engineered mutation
Gln 84	Lys 1589	conflict
Arg 104	Lys 1609	conflict
Lys 179	-	cloning artifact
Gly 180	-	cloning artifact
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	26016.9
All*	Total formula weight	26016.9

*Water molecules are not included.