2GFY

Structure of E. coli FabF(K335A) mutant with covalently linked dodecanoic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	3-oxoacyl-[acyl-carrier-protein] synthase 2	polymer	427	44579.2	1	UniProt (P0AAI5) Pfam (PF00109) Pfam (PF02801)	Escherichia coli	3-oxoacyl-[acyl-carrier-protein] synthase II; Beta-ketoacyl-ACP synthase II; KAS II; FabF
2	B (A)	LAURIC ACID	non-polymer		200.3	1	Chemie (DAO)
3	C (A)	water	water		18.0	76	Chemie (HOH)

Sequence modifications

A: 1 - 412 (UniProt: P0AAI5)

PDB	External Database	Details
Met -14	-	expression tag
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Ala -2	-	expression tag
Cys -1	-	expression tag
Val 0	-	expression tag
Ala 335	Lys 335	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44579.2
Non-Polymers*	Number of molecules	1
	Total formula weight	200.3
All*	Total formula weight	44779.5

*Water molecules are not included.