2GAF

Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase	polymer	297	35057.9	1	UniProt (P07617) Pfam (PF01358) UniProt (by SIFTS) (Q1PIV4) In PDB	Vaccinia virus	PolyA, polymerase regulatory subunit, PolyA, polymerase small subunit, PAP-S, VP39
2	D	Poly(A) polymerase catalytic subunit	polymer	469	54388.8	1	UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629) In PDB	Vaccinia virus	PolyA, polymerase large subunit, PAP-L, VP55
3		water	water		18.0	140	Chemie (HOH)

Sequence modifications

A: 1 - 297 (UniProt: P07617)

PDB	External Database	Details
Mse 1	Met 1	modified residue
Mse 11	Met 11	modified residue
Mse 92	Met 92	modified residue
Ala 140	Arg 140	engineered mutation
Ala 142	Lys 142	engineered mutation
Ala 143	Arg 143	engineered mutation
Mse 163	Met 163	modified residue
Mse 196	Met 196	modified residue
Mse 208	Met 208	modified residue
Mse 219	Met 219	modified residue
Mse 236	Met 236	modified residue
Mse 263	Met 263	modified residue
Mse 266	Met 266	modified residue

D: 11 - 479 (UniProt: P23371)

PDB	External Database	Details
Ser 36	Leu 36	variant

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89446.8
All*	Total formula weight	89446.8

*Water molecules are not included.