2GAF
Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-11-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9791, 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.085, 91.260, 133.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.400 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.260 |
| Number of reflections | 33681 | |
| Completeness [%] | 83.4 | 68.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 20% PEG 4000, 0.24 M CaCl2, 5% glycerol, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
