2GA9
Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase | polymer | 297 | 34589.0 | 1 | UniProt (P07617) Pfam (PF01358) | Vaccinia virus | PolyA, polymerase regulatory subunit, PolyA, polymerase small subunit, PAP-S, VP39 |
| 2 | B (D) | Poly(A) polymerase catalytic subunit | polymer | 469 | 54388.8 | 1 | UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629) | Vaccinia virus | PolyA, polymerase large subunit, PAP-L, VP55 |
| 3 | C, D (D) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | E, F (D) | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 2 | Chemie (AGS) | |||
| 5 | G, H (A, D) | water | water | 18.0 | 260 | Chemie (HOH) |
Sequence modifications
A: 1 - 297 (UniProt: P07617)
D: 11 - 479 (UniProt: P23371)
| PDB | External Database | Details |
|---|---|---|
| Ala 140 | Arg 140 | engineered mutation |
| Ala 142 | Lys 142 | engineered mutation |
| Ala 143 | Arg 143 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 36 | Leu 36 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 88977.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1126.6 | |
| All* | Total formula weight | 90104.5 |
