2GA9

Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase	polymer	297	34589.0	1	UniProt (P07617) Pfam (PF01358) In PDB	Vaccinia virus	PolyA, polymerase regulatory subunit, PolyA, polymerase small subunit, PAP-S, VP39
2	D	Poly(A) polymerase catalytic subunit	polymer	469	54388.8	1	UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629) In PDB	Vaccinia virus	PolyA, polymerase large subunit, PAP-L, VP55
3	D	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	D	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
5		water	water		18.0	260	Chemie (HOH)

Sequence modifications

A: 1 - 297 (UniProt: P07617)

PDB	External Database	Details
Ala 140	Arg 140	engineered mutation
Ala 142	Lys 142	engineered mutation
Ala 143	Arg 143	engineered mutation

D: 11 - 479 (UniProt: P23371)

PDB	External Database	Details
Ser 36	Leu 36	variant

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88977.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1126.6
All*	Total formula weight	90104.5

*Water molecules are not included.