2G77
Crystal Structure of Gyp1 TBC domain in complex with Rab33 GTPase bound to GDP and AlF3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | GTPase-activating protein GYP1 | polymer | 410 | 48158.5 | 1 | UniProt (Q08484) Pfam (PF00566) | Saccharomyces cerevisiae (baker's yeast) | GAP for YPT1 |
| 2 | B (B) | Ras-related protein Rab-33B | polymer | 198 | 22549.1 | 1 | UniProt (O35963) Pfam (PF00071) | Mus musculus (house mouse) | |
| 3 | C (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | D (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 5 | E (B) | ALUMINUM FLUORIDE | non-polymer | 84.0 | 1 | Chemie (AF3) | |||
| 6 | F, G (A, B) | water | water | 18.0 | 416 | Chemie (HOH) |
Sequence modifications
A: 244 - 637 (UniProt: Q08484)
B: 14 - 202 (UniProt: O35963)
| PDB | External Database | Details |
|---|---|---|
| Met 228 | - | cloning artifact |
| Gly 229 | - | cloning artifact |
| His 230 | - | cloning artifact |
| His 231 | - | cloning artifact |
| His 232 | - | cloning artifact |
| His 233 | - | cloning artifact |
| His 234 | - | cloning artifact |
| His 235 | - | cloning artifact |
| Gly 236 | - | cloning artifact |
| Ser 237 | - | cloning artifact |
| Leu 238 | - | cloning artifact |
| Val 239 | - | cloning artifact |
| Pro 240 | - | cloning artifact |
| Arg 241 | - | cloning artifact |
| Gly 242 | - | cloning artifact |
| Ser 243 | - | cloning artifact |
| Lys 405 | Glu 405 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 5 | - | cloning artifact |
| Gly 6 | - | cloning artifact |
| His 7 | - | cloning artifact |
| His 8 | - | cloning artifact |
| His 9 | - | cloning artifact |
| His 10 | - | cloning artifact |
| His 11 | - | cloning artifact |
| His 12 | - | cloning artifact |
| Gly 13 | - | cloning artifact |
| Mse 116 | Met 116 | modified residue |
| Mse 119 | Met 119 | modified residue |
| Mse 172 | Met 172 | modified residue |
| Mse 193 | Met 193 | modified residue |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 70707.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 551.5 | |
| All* | Total formula weight | 71259.1 |
