2G77

Crystal Structure of Gyp1 TBC domain in complex with Rab33 GTPase bound to GDP and AlF3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	GTPase-activating protein GYP1	polymer	410	48158.5	1	UniProt (Q08484) Pfam (PF00566) In PDB	Saccharomyces cerevisiae (baker's yeast)	GAP for YPT1
2	B	Ras-related protein Rab-33B	polymer	198	22549.1	1	UniProt (O35963) Pfam (PF00071) In PDB	Mus musculus (house mouse)
3	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
5	B	ALUMINUM FLUORIDE	non-polymer		84.0	1	Chemie (AF3)
6		water	water		18.0	416	Chemie (HOH)

Sequence modifications

A: 244 - 637 (UniProt: Q08484)

PDB	External Database	Details
Met 228	-	cloning artifact
Gly 229	-	cloning artifact
His 230	-	cloning artifact
His 231	-	cloning artifact
His 232	-	cloning artifact
His 233	-	cloning artifact
His 234	-	cloning artifact
His 235	-	cloning artifact
Gly 236	-	cloning artifact
Ser 237	-	cloning artifact
Leu 238	-	cloning artifact
Val 239	-	cloning artifact
Pro 240	-	cloning artifact
Arg 241	-	cloning artifact
Gly 242	-	cloning artifact
Ser 243	-	cloning artifact
Lys 405	Glu 405	engineered mutation

B: 14 - 202 (UniProt: O35963)

PDB	External Database	Details
Met 5	-	cloning artifact
Gly 6	-	cloning artifact
His 7	-	cloning artifact
His 8	-	cloning artifact
His 9	-	cloning artifact
His 10	-	cloning artifact
His 11	-	cloning artifact
His 12	-	cloning artifact
Gly 13	-	cloning artifact
Mse 116	Met 116	modified residue
Mse 119	Met 119	modified residue
Mse 172	Met 172	modified residue
Mse 193	Met 193	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70707.6
Non-Polymers*	Number of molecules	3
	Total formula weight	551.5
All*	Total formula weight	71259.1

*Water molecules are not included.