2G77
Crystal Structure of Gyp1 TBC domain in complex with Rab33 GTPase bound to GDP and AlF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979026 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.200, 94.526, 102.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.260 |
R-factor | 0.20726 |
Rwork | 0.205 |
R-free | 0.24357 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.987 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 33046 | |
<I/σ(I)> | 9.9 | 4.93 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 277 | 10~15% polyethylene glycol 6000, 100 mM Sodium Acetate, 10% Glycerol, 20 mM NaF, 2mM AlCl3, 50 mM HEPES, pH7.0, EVAPORATION, temperature 277K |
1 | EVAPORATION | 7 | 277 | 10~15% polyethylene glycol 6000, 100 mM Sodium Acetate, 10% Glycerol, 20 mM NaF, 2mM AlCl3, 50 mM HEPES, pH7.0, EVAPORATION, temperature 277K |