2G54

Crystal structure of Zn-bound human insulin-degrading enzyme in complex with insulin B chain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Insulin-degrading enzyme	polymer	990	114715.1	2	UniProt (Q5T5N2) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) UniProt (by SIFTS) (P14735)	Homo sapiens (human)	Insulysin, Insulinase, Insulin protease
2	C, D (C, D)	insulin	polymer	30	3434.0	2	UniProt (P01308) Pfam (PF00049)
3	E, G (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, H (A, B)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
5	I, J, K, L (A, B, C, D)	water	water		18.0	787	Chemie (HOH)

Sequence modifications

A, B: 42 - 1019 (UniProt: Q5T5N2)

PDB	External Database	Details
Met 30	-	initiating methionine
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Ala 37	-	cloning artifact
Ala 38	-	cloning artifact
Gly 39	-	cloning artifact
Ile 40	-	cloning artifact
Pro 41	-	cloning artifact
Gln 111	Glu 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	236298.2
Non-Polymers*	Number of molecules	4
	Total formula weight	307.0
All*	Total formula weight	236605.2

*Water molecules are not included.