2FYN

Crystal Structure Analysis of the double mutant Rhodobacter Sphaeroides bc1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J, M...	Cytochrome b	polymer	445	50157.5	6	UniProt (Q02761) Pfam (PF00033) Pfam (PF00032) In PDB	Rhodobacter sphaeroides
2	B, E, H, K, N...	Cytochrome c1	polymer	269	29374.0	6	UniProt (Q02760) Pfam (PF02167) In PDB	Rhodobacter sphaeroides
3	C, F, I, L, O...	Ubiquinol-cytochrome c reductase iron-sulfur subunit	polymer	187	19916.3	6	UniProt (Q02762) Pfam (PF10399) Pfam (PF00355) In PDB	Rhodobacter sphaeroides	Rieske iron-sulfur protein, RISP
4	Q, E, G, H, J...	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	18	Chemie (HEM)
5	D, G, J, M, A...	STIGMATELLIN A	non-polymer		514.6	6	Chemie (SMA)
6	D, G, J, M, A...	(1R)-2-{[(R)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(DODECANOYLOXY)METHYL]ETHYL (9Z)-OCTADEC-9-ENOATE	non-polymer		661.9	6	Chemie (LOP)
7	R, F, I, L, O...	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	6	Chemie (FES)

Sequence modifications

A, D, G, J, M, P: 2 - 445 (UniProt: Q02761)

PDB	External Database	Details
Met 1	-	initiating methionine
Arg 287	Ser 286	engineered mutation

B, E, H, K, N, Q: 1 - 263 (UniProt: Q02760)

PDB	External Database	Details
Pro 98	Ala 120	conflict
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	insertion
His 269	-	insertion

C, F, I, L, O, R: 1 - 187 (UniProt: Q02762)

PDB	External Database	Details
Ser 135	Val 135	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	596686.9
Non-Polymers*	Number of molecules	36
	Total formula weight	19210.9
All*	Total formula weight	615897.8

*Water molecules are not included.