2FYD
catalytic domain of bovine beta 1, 4-galactosyltransferase in complex with alpha-lactalbumin, glucose, Mn, and UDP-N-acetylgalactosamine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Alpha-lactalbumin | polymer | 123 | 14015.8 | 2 | UniProt (P29752) Pfam (PF00062) | Mus musculus (house mouse) | Lactose synthase B protein |
| 2 | B, D (B, D) | beta-1,4-galactosyltransferase | polymer | 286 | 32772.5 | 2 | GenBank (21450879) Pfam (PF13733) Pfam (PF02709) UniProt (by SIFTS) (P08037) | Bos taurus (cattle) | |
| 3 | E, L (A, C) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) PubChem (271) | |||
| 4 | F, M (A, C) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) PubChem (8200) | |||
| 5 | G, N (A, C) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) PubChem (4478249) | |||
| 6 | H, O (B, D) | beta-D-glucopyranose | non-polymer | 180.2 | 2 | Chemie (BGC) | |||
| 7 | I, P (B, D) | 2-acetamido-2-deoxy-beta-D-galactopyranose | non-polymer | 221.2 | 2 | Chemie (NGA) | |||
| 8 | J, Q (B, D) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) PubChem (27854) | |||
| 9 | K, R (B, D) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 2 | Chemie (UDP) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (57448939) PubChem (53486216) PubChem (60098857) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (1158) PubChem (6031) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241) | |||
| 10 | S, T, U, V (A, B, C, D) | water | water | 18.0 | 509 | Chemie (HOH) |
Sequence modifications
B, D: 130 - 402 (GenBank: 21450879)
| PDB | External Database | Details |
|---|---|---|
| Ala 117 | - | cloning artifact |
| Ser 118 | - | cloning artifact |
| Met 119 | - | cloning artifact |
| Thr 120 | - | cloning artifact |
| Gly 121 | - | cloning artifact |
| Gly 122 | - | cloning artifact |
| Gln 123 | - | cloning artifact |
| Gln 124 | - | cloning artifact |
| Met 125 | - | cloning artifact |
| Gly 126 | - | cloning artifact |
| Arg 127 | - | cloning artifact |
| Gly 128 | - | cloning artifact |
| Ser 129 | - | cloning artifact |
| Cys 312 | Trp 239 | engineered mutation |
| Thr 342 | Cys 269 | engineered mutation |
| Cys 401 | Pro 328 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 93576.7 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 2580.0 | |
| All* | Total formula weight | 96156.8 |
