2FO5

Crystal structure of recombinant barley cysteine endoprotease B isoform 2 (EP-B2) in complex with leupeptin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Cysteine proteinase EP-B 2	polymer	262	27759.7	4	UniProt (P25250) Pfam (PF00112) In PDB	Hordeum vulgare
2	E, F, G, H	ACE-LEU-LEU-argininal (leupeptin)	polymer	4	429.6	4	NOR (NOR00487) BIRD (PRD_000216)	Actinomycetes Streptomyces roseus MA 839-A1
3	A, B, C, D	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
4		water	water		18.0	509	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 243 (UniProt: P25250)

PDB	External Database	Details
Asn 244	-	EXPRESSION TAG
Ser 245	-	EXPRESSION TAG
Ser 246	-	EXPRESSION TAG
Ser 247	-	EXPRESSION TAG
Val 248	-	EXPRESSION TAG
Asp 249	-	EXPRESSION TAG
Lys 250	-	EXPRESSION TAG
Leu 251	-	EXPRESSION TAG
Ala 252	-	EXPRESSION TAG
Ala 253	-	EXPRESSION TAG
Ala 254	-	EXPRESSION TAG
Leu 255	-	EXPRESSION TAG
Glu 256	-	EXPRESSION TAG
His 257	-	EXPRESSION TAG
His 258	-	EXPRESSION TAG
His 259	-	EXPRESSION TAG
His 260	-	EXPRESSION TAG
His 261	-	EXPRESSION TAG
His 262	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	112757.1
Non-Polymers*	Number of molecules	9
	Total formula weight	864.6
All*	Total formula weight	113621.7

*Water molecules are not included.