2FLD

I-MsoI Re-Designed for Altered DNA Cleavage Specificity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	5'-D(*GP*CP*AP*GP*AP*AP*GP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*CP*GP*TP*TP*CP*CP*G)-3'	polymer	24	7435.8	1
2	D	5'-D(*CP*GP*GP*AP*AP*CP*GP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*CP*TP*TP*CP*TP*GP*C)-3'	polymer	24	7306.7	1
3	A, B	DNA ENDONUCLEASE I-MSOI	polymer	165	18934.8	2	UniProt (Q8WKW7) Pfam (PF00961) UniProt (by SIFTS) (C0JWR6) In PDB	Monomastix sp.
4	C	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6		water	water		18.0	97	Chemie (HOH)

Sequence modifications

A, B: 6 - 170 (UniProt: Q8WKW7)

PDB	External Database	Details
Leu 28	Lys 28	engineered mutation
Arg 83	Thr 83	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52612.1
Non-Polymers*	Number of molecules	3
	Total formula weight	103.1
All*	Total formula weight	52715.2

*Water molecules are not included.