2FLD
I-MsoI Re-Designed for Altered DNA Cleavage Specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-01 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 |
Unit cell lengths | 41.961, 42.981, 71.545 |
Unit cell angles | 72.29, 72.30, 70.51 |
Refinement procedure
Resolution | 39.500 - 2.000 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m5x MUTATED RESIDUES EXCLUDED |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.500 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.079 | 0.338 |
Number of reflections | 27855 | |
<I/σ(I)> | 22.3 | 3 |
Completeness [%] | 94.1 | 74.2 |
Redundancy | 3.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | 30% PEG 400, 100 mM Tris, 50 mM NaCl, 10 mM CaCl2, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 30% PEG 400, 100 mM Tris,50 mM NaCl, 10 mM CaCl2 | ||
2 | 1 | 2 | 30% PEG 400, 100 mM Tris,50 mM NaCl, 10 mM CaCl2 |