2FGE

Crystal structure of presequence protease PreP from Arabidopsis thaliana

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	zinc metalloprotease (insulinase family)	polymer	995	112536.4	2	UniProt (Q9LJL3) Pfam (PF00675) Pfam (PF05193) Pfam (PF08367) Pfam (PF22516)	Arabidopsis thaliana (thale cress)	Presequence protease PreP; ATPREP2; metalloendopeptidase; ATPREP1/ATZNMP;
2	C, D (D, E)	nonspecific peptide AALTRA	polymer	6	602.7	2
3	E, I (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, J (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	G, H, K, L (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	M, N, O (A, B, D)	water	water		18.0	948	Chemie (HOH)

Sequence modifications

A, B: 1 - 995 (UniProt: Q9LJL3)

PDB	External Database	Details
Mse 50	Met 107	modified residue
Gln 80	Glu 137	engineered mutation
Mse 178	Met 235	modified residue
Mse 258	Met 315	modified residue
Mse 299	Met 356	modified residue
Mse 326	Met 383	modified residue
Mse 381	Met 438	modified residue
Mse 401	Met 458	modified residue
Mse 423	Met 480	modified residue
Mse 434	Met 491	modified residue
Mse 481	Met 538	modified residue
Mse 506	Met 563	modified residue
Mse 604	Met 661	modified residue
Mse 651	Met 708	modified residue
Mse 662	Met 719	modified residue
Mse 688	Met 745	modified residue
Mse 704	Met 761	modified residue
Mse 707	Met 764	modified residue
Mse 714	Met 771	modified residue
Mse 718	Met 775	modified residue
Mse 762	Met 819	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	226278.1
Non-Polymers*	Number of molecules	8
	Total formula weight	298.9
All*	Total formula weight	226577.1

*Water molecules are not included.