2F9P
Crystal Structure of the Recombinant Human Alpha I Tryptase Mutant D216G in Complex with Leupeptin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, B, C, D) | Tryptase alpha-1 | polymer | 245 | 27660.7 | 4 | GenBank (1182067) Pfam (PF00089) UniProt (by SIFTS) (Q15661) | Homo sapiens (human) | Tryptase-1 |
| 2 | B, D, F, H (E, F, G, H) | Leupeptin | polymer | 4 | 429.6 | 4 | NOR (NOR00487) BIRD (PRD_000216) | Actinomycetes Streptomyces roseus MA 839-A1 | |
| 3 | I (I) | alpha-L-fucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 1 | In PDB GlyTouCan (G80587NA) | |||
| 4 | J, L, M, N (A, B, C, D) | (R,R)-2,3-BUTANEDIOL | non-polymer | 90.1 | 4 | Chemie (BU3) | |||
| 5 | K (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 6 | O, P, Q, R, S... (A, E, B, F, C...) | water | water | 18.0 | 502 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 16 - 246 (GenBank: 1182067)
| PDB | External Database | Details |
|---|---|---|
| Val 59 | Leu 76 | engineered mutation |
| Gly 216 | Asp 245 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 112361.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 367.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 581.7 | |
| All* | Total formula weight | 113310.1 |
