2F9N
Crystal Structure of the Recombinant Human Alpha I Tryptase Mutant K192Q/D216G in Complex with Leupeptin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | alpha I tryptase | polymer | 245 | 27659.6 | 4 | UniProt (P15157) Pfam (PF00089) UniProt (by SIFTS) (Q15661) In PDB | Homo sapiens (human) | Tryptase-1, Tryptase alpha-1 |
| 2 | E, F, G, H | Leupeptin | polymer | 4 | 429.6 | 4 | NOR (NOR00487) BIRD (PRD_000216) | Actinomycetes Streptomyces roseus MA 839-A1 | |
| 3 | I, J | alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 716.7 | 2 | In PDB GlyTouCan (G63564LA) | |||
| 4 | A, B, C, D | (R,R)-2,3-BUTANEDIOL | non-polymer | 90.1 | 4 | Chemie (BU3) | |||
| 5 | B, D | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 6 | water | water | 18.0 | 1178 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 16 - 246 (UniProt: P15157)
| PDB | External Database | Details |
|---|---|---|
| Val 59 | Leu 76 | engineered mutation |
| Ser 186 | Thr 215 | variant |
| Gln 187 | Arg 216 | variant |
| Gln 192 | Lys 221 | engineered mutation |
| Gly 216 | Asp 245 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 112356.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1433.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 802.9 | |
| All* | Total formula weight | 114593.1 |
