2EA0
Crystal structure of the DNA repair enzyme endonuclease-VIII (Nei) from E. coli in complex with AP-site containing DNA substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B | 5'-D(P*GP*GP*CP*TP*TP*CP*AP*TP*CP*CP*TP*G)-3' | polymer | 12 | 3629.4 | 1 | |||
| 2 | C | 5'-D(P*CP*AP*GP*GP*AP*(PED)P*GP*AP*AP*GP*CP*C)-3' | polymer | 12 | 3574.3 | 1 | |||
| 3 | A | Endonuclease VIII | polymer | 262 | 29815.0 | 1 | UniProt (P50465) Pfam (PF01149) Pfam (PF06831) Pfam (PF06827) In PDB | Escherichia coli | DNA glycosylase/AP lyase Nei, DNA-apurinic or apyrimidinic site, lyase Nei |
| 4 | C, A | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 5 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | water | water | 18.0 | 412 | Chemie (HOH) |
Sequence modifications
A: 1 - 262 (UniProt: P50465)
| PDB | External Database | Details |
|---|---|---|
| Thr 34 | Pro 35 | SEE REMARK 999 |
| Arg 112 | Thr 113 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 37018.7 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 637.8 | |
| All* | Total formula weight | 37656.5 |
