2E41

Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with the Reaction Product Analog Biotinol-5'-AMP, Mutations R48A and K111A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	biotin--[acetyl-CoA-carboxylase] ligase	polymer	235	25959.3	2	UniProt (O57883) Pfam (PF03099) Pfam (PF02237)	Pyrococcus horikoshii	Biotin Protein Ligase
2	C, D (A, B)	((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHYL 5-((3AS,4S,6AR)-2-OXO-HEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL)PENTYL HYDROGEN PHOSPHATE	non-polymer		559.5	2	Chemie (BTX)
3	E, F (A, B)	water	water		18.0	501	Chemie (HOH)

Sequence modifications

A, B: 1 - 235 (UniProt: O57883)

PDB	External Database	Details
Ala 48	Arg 48	engineered mutation
Ala 111	Lys 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51918.6
Non-Polymers*	Number of molecules	2
	Total formula weight	1119.1
All*	Total formula weight	53037.7

*Water molecules are not included.