2DET

Cocrystal structure of an RNA sulfuration enzyme MnmA and tRNA-Glu in the pre-reaction state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	tRNA	polymer	76	24378.5	1
2	A	tRNA-specific 2-thiouridylase mnmA	polymer	380	42411.8	1	UniProt (P25745) Pfam (PF03054) Pfam (PF20259) Pfam (PF20258) In PDB	Escherichia coli	RNA sulfuration enzyme MnmA
3	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)

Sequence modifications

A: 1 - 368 (UniProt: P25745)

PDB	External Database	Details
Met -11	-	EXPRESSION TAG
Arg -10	-	EXPRESSION TAG
Gly -9	-	EXPRESSION TAG
Ser -8	-	EXPRESSION TAG
His -7	-	EXPRESSION TAG
His -6	-	EXPRESSION TAG
His -5	-	EXPRESSION TAG
His -4	-	EXPRESSION TAG
His -3	-	EXPRESSION TAG
His -2	-	EXPRESSION TAG
Gly -1	-	EXPRESSION TAG
Ser 0	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66790.3
Non-Polymers*	Number of molecules	1
	Total formula weight	96.1
All*	Total formula weight	66886.4

*Water molecules are not included.