2DET

Cocrystal structure of an RNA sulfuration enzyme MnmA and tRNA-Glu in the pre-reaction state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0002143	biological_process	tRNA wobble position uridine thiolation
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006400	biological_process	tRNA modification
A	0008033	biological_process	tRNA processing
A	0016740	molecular_function	transferase activity
A	0016783	molecular_function	sulfurtransferase activity
A	0103016	molecular_function	tRNA-uridine 2-sulfurtransferase activity
A	1990228	cellular_component	sulfurtransferase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 5001

Chain	Residue
A	SER13
A	GLY14
A	GLY15
A	ASP17
A	SER18

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Functional Information from PROSITE/UniProt

site_id	PS00070
Number of Residues	12
Details	ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. VfYNGEVCLGGG

Chain	Residue	Details
A	VAL347-GLY358

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:16871210

Chain	Residue	Details
A	CYS102

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000269|PubMed:16871210

Chain	Residue	Details
A	CYS199

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site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	GLY11
A	MET37
A	GLY127

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site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Interaction with tRNA

Chain	Residue	Details
A	HIS128
A	GLN344

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1kp2

Chain	Residue	Details
A	ASP136
A	LYS104

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