2DER

Cocrystal structure of an RNA sulfuration enzyme MnmA and tRNA-Glu in the initial tRNA binding state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, D	tRNA	polymer	76	24378.5	2
2	A, B	tRNA-specific 2-thiouridylase mnmA	polymer	380	42411.8	2	UniProt (P25745) Pfam (PF03054) Pfam (PF20259) Pfam (PF20258) In PDB	Escherichia coli	RNA sulfuration enzyme MnmA
3	C, A, B	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
4	A, B	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)

Sequence modifications

A, B: 1 - 368 (UniProt: P25745)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	133580.7
Non-Polymers*	Number of molecules	9
	Total formula weight	860.2
All*	Total formula weight	134440.9

*Water molecules are not included.