2D74

Crystal structure of translation initiation factor aIF2betagamma heterodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Translation initiation factor 2 gamma subunit	polymer	419	46229.5	1	UniProt (Q8U082) Pfam (PF00009) Pfam (PF03144) Pfam (PF09173) In PDB	Pyrococcus furiosus	eIF-2-gamma, aIF2-gamma
2	B	Translation initiation factor 2 beta subunit	polymer	148	17332.1	1	UniProt (Q8U3I5) Pfam (PF01873) In PDB	Pyrococcus furiosus	eIF-2-beta, aIF2-beta
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4		water	water		18.0	180	Chemie (HOH)

Sequence modifications

A: 1 - 411 (UniProt: Q8U082)

PDB	External Database	Details
Asp 236	Gly 236	engineered mutation
Leu 412	-	expression tag
Glu 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag

B: 1 - 140 (UniProt: Q8U3I5)

PDB	External Database	Details
Leu 141	-	expression tag
Glu 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	63561.5
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	63692.4

*Water molecules are not included.