2CBM

Crystal structure of the apo-form of a neocarzinostatin mutant evolved to bind testosterone.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NEOCARZINOSTATIN	polymer	112	11225.2	1	UniProt (P0A3R9) Pfam (PF00960) In PDB	STREPTOMYCES CARZINOSTATICUS	NCS, MITOMALCIN, MMC
2	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
3		water	water		18.0	54	Chemie (HOH)

Sequence modifications

A: 1 - 112 (UniProt: P0A3R9)

PDB	External Database	Details
Trp 33	Asp 67	engineered mutation
Ala 35	Gly 69	engineered mutation
Trp 37	Cys 71	engineered mutation
Arg 39	Trp 73	engineered mutation
Trp 45	Leu 79	engineered mutation
Tyr 47	Cys 81	engineered mutation
Asn 52	Phe 86	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	11225.2
Non-Polymers*	Number of molecules	1
	Total formula weight	195.2
All*	Total formula weight	11420.4

*Water molecules are not included.