2C5L

Structure of PLC epsilon Ras association domain with hRas

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GTPASE HRAS	polymer	173	19376.7	2	UniProt (P01112) Pfam (PF00071)	HOMO SAPIENS (HUMAN)	TRANSFORMING PROTEIN P21, H-RAS-1, C-H-RAS
2	C, D (C, D)	PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C PLC-EPSILON	polymer	117	13157.9	2	UniProt (Q9HBX6) Pfam (PF00788) UniProt (by SIFTS) (Q9P212)	HOMO SAPIENS (HUMAN)
3	E, I (A, B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
4	F, J (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
5	G, H, K, L (A, B, C)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL) PubChem (753)
6	M, N, O, P (A, B, C, D)	water	water		18.0	476	Chemie (HOH)

Sequence modifications

A, B: -6 - 0 (PDB: 2C5L)
A, B: 1 - 166 (UniProt: P01112)

PDB	External Database	Details
Val 12	Gly 12	engineered mutation

C, D: 2130 - 2130 (PDB: 2C5L)
C, D: 2131 - 2246 (UniProt: Q9HBX6)

PDB	External Database	Details
Leu 2176	Tyr 2176	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65069.1
Non-Polymers*	Number of molecules	8
	Total formula weight	1463.3
All*	Total formula weight	66532.4

*Water molecules are not included.