2C2V

Crystal structure of the CHIP-UBC13-UEV1a complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, E, H, K	Ubiquitin-conjugating enzyme E2 N	polymer	154	17241.8	4	UniProt (P61088) Pfam (PF00179) In PDB	Homo sapiens (Human)	Bendless-like ubiquitin-conjugating enzyme,E2 ubiquitin-conjugating enzyme N,Ubc13,UbcH13,Ubiquitin carrier protein N,Ubiquitin-protein ligase N
2	C, F, I, L	Ubiquitin-conjugating enzyme E2 variant 1	polymer	142	16095.5	4	UniProt (Q13404) Pfam (PF00179) In PDB	Homo sapiens (Human)	UEV-1,CROC-1,TRAF6-regulated IKK activator 1 beta Uev1A
3	S, T, U, V	STIP1 homology and U box-containing protein 1	polymer	78	9033.2	4	UniProt (Q9WUD1) Pfam (PF04564) In PDB	Mus musculus (Mouse)	Carboxy terminus of Hsp70-interacting protein,E3 ubiquitin-protein ligase CHIP,RING-type E3 ubiquitin transferase CHIP
4		water	water		18.0	35	Chemie (HOH)

Sequence modifications

B, E, H, K: 6 - 156 (UniProt: P61088)

PDB	External Database	Details
Ala 3	-	expression tag
Gly 4	-	expression tag
Ser 5	-	expression tag

C, F, I, L: 33 - 174 (UniProt: Q13404)

PDB	External Database	Details
Leu 80	Ile 127	conflict

S, T, U, V: 227 - 304 (UniProt: Q9WUD1)

PDB	External Database	Details
Asn 269	Asp 269	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	169481.7
All*	Total formula weight	169481.7

*Water molecules are not included.