2C2V

Crystal structure of the CHIP-UBC13-UEV1a complex

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000151	cellular_component	ubiquitin ligase complex
B	0000166	molecular_function	nucleotide binding
B	0000209	biological_process	protein polyubiquitination
B	0000724	biological_process	double-strand break repair via homologous recombination
B	0000729	biological_process	DNA double-strand break processing
B	0003723	molecular_function	RNA binding
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006281	biological_process	DNA repair
B	0006282	biological_process	regulation of DNA repair
B	0006301	biological_process	DNA damage tolerance
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0006513	biological_process	protein monoubiquitination
B	0006974	biological_process	DNA damage response
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0031371	cellular_component	ubiquitin conjugating enzyme complex
B	0031372	cellular_component	UBC13-MMS2 complex
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0032991	cellular_component	protein-containing complex
B	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
B	0043130	molecular_function	ubiquitin binding
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0045739	biological_process	positive regulation of DNA repair
B	0050852	biological_process	T cell receptor signaling pathway
B	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
B	0061631	molecular_function	ubiquitin conjugating enzyme activity
B	0070062	cellular_component	extracellular exosome
B	0070534	biological_process	protein K63-linked ubiquitination
B	0097027	molecular_function	ubiquitin-protein transferase activator activity
B	0140374	biological_process	antiviral innate immune response
B	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
B	1902533	biological_process	positive regulation of intracellular signal transduction
B	1904262	biological_process	negative regulation of TORC1 signaling
B	2000781	biological_process	positive regulation of double-strand break repair
C	0000151	cellular_component	ubiquitin ligase complex
C	0000209	biological_process	protein polyubiquitination
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006282	biological_process	regulation of DNA repair
C	0006301	biological_process	DNA damage tolerance
C	0006355	biological_process	regulation of DNA-templated transcription
C	0030154	biological_process	cell differentiation
C	0031371	cellular_component	ubiquitin conjugating enzyme complex
C	0031372	cellular_component	UBC13-MMS2 complex
C	0031624	molecular_function	ubiquitin conjugating enzyme binding
C	0032991	cellular_component	protein-containing complex
C	0042275	biological_process	error-free postreplication DNA repair
C	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0061631	molecular_function	ubiquitin conjugating enzyme activity
C	0070062	cellular_component	extracellular exosome
C	0070534	biological_process	protein K63-linked ubiquitination
C	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
C	1902533	biological_process	positive regulation of intracellular signal transduction
E	0000151	cellular_component	ubiquitin ligase complex
E	0000166	molecular_function	nucleotide binding
E	0000209	biological_process	protein polyubiquitination
E	0000724	biological_process	double-strand break repair via homologous recombination
E	0000729	biological_process	DNA double-strand break processing
E	0003723	molecular_function	RNA binding
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006282	biological_process	regulation of DNA repair
E	0006301	biological_process	DNA damage tolerance
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0006513	biological_process	protein monoubiquitination
E	0006974	biological_process	DNA damage response
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0031371	cellular_component	ubiquitin conjugating enzyme complex
E	0031372	cellular_component	UBC13-MMS2 complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0032991	cellular_component	protein-containing complex
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043130	molecular_function	ubiquitin binding
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0045739	biological_process	positive regulation of DNA repair
E	0050852	biological_process	T cell receptor signaling pathway
E	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
E	0061631	molecular_function	ubiquitin conjugating enzyme activity
E	0070062	cellular_component	extracellular exosome
E	0070534	biological_process	protein K63-linked ubiquitination
E	0097027	molecular_function	ubiquitin-protein transferase activator activity
E	0140374	biological_process	antiviral innate immune response
E	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
E	1902533	biological_process	positive regulation of intracellular signal transduction
E	1904262	biological_process	negative regulation of TORC1 signaling
E	2000781	biological_process	positive regulation of double-strand break repair
F	0000151	cellular_component	ubiquitin ligase complex
F	0000209	biological_process	protein polyubiquitination
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006282	biological_process	regulation of DNA repair
F	0006301	biological_process	DNA damage tolerance
F	0006355	biological_process	regulation of DNA-templated transcription
F	0030154	biological_process	cell differentiation
F	0031371	cellular_component	ubiquitin conjugating enzyme complex
F	0031372	cellular_component	UBC13-MMS2 complex
F	0031624	molecular_function	ubiquitin conjugating enzyme binding
F	0032991	cellular_component	protein-containing complex
F	0042275	biological_process	error-free postreplication DNA repair
F	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
F	0045893	biological_process	positive regulation of DNA-templated transcription
F	0061631	molecular_function	ubiquitin conjugating enzyme activity
F	0070062	cellular_component	extracellular exosome
F	0070534	biological_process	protein K63-linked ubiquitination
F	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
F	1902533	biological_process	positive regulation of intracellular signal transduction
H	0000151	cellular_component	ubiquitin ligase complex
H	0000166	molecular_function	nucleotide binding
H	0000209	biological_process	protein polyubiquitination
H	0000724	biological_process	double-strand break repair via homologous recombination
H	0000729	biological_process	DNA double-strand break processing
H	0003723	molecular_function	RNA binding
H	0004842	molecular_function	ubiquitin-protein transferase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006281	biological_process	DNA repair
H	0006282	biological_process	regulation of DNA repair
H	0006301	biological_process	DNA damage tolerance
H	0006511	biological_process	ubiquitin-dependent protein catabolic process
H	0006513	biological_process	protein monoubiquitination
H	0006974	biological_process	DNA damage response
H	0016567	biological_process	protein ubiquitination
H	0016740	molecular_function	transferase activity
H	0031371	cellular_component	ubiquitin conjugating enzyme complex
H	0031372	cellular_component	UBC13-MMS2 complex
H	0031625	molecular_function	ubiquitin protein ligase binding
H	0032991	cellular_component	protein-containing complex
H	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
H	0043130	molecular_function	ubiquitin binding
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0045739	biological_process	positive regulation of DNA repair
H	0050852	biological_process	T cell receptor signaling pathway
H	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
H	0061631	molecular_function	ubiquitin conjugating enzyme activity
H	0070062	cellular_component	extracellular exosome
H	0070534	biological_process	protein K63-linked ubiquitination
H	0097027	molecular_function	ubiquitin-protein transferase activator activity
H	0140374	biological_process	antiviral innate immune response
H	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
H	1902533	biological_process	positive regulation of intracellular signal transduction
H	1904262	biological_process	negative regulation of TORC1 signaling
H	2000781	biological_process	positive regulation of double-strand break repair
I	0000151	cellular_component	ubiquitin ligase complex
I	0000209	biological_process	protein polyubiquitination
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006282	biological_process	regulation of DNA repair
I	0006301	biological_process	DNA damage tolerance
I	0006355	biological_process	regulation of DNA-templated transcription
I	0030154	biological_process	cell differentiation
I	0031371	cellular_component	ubiquitin conjugating enzyme complex
I	0031372	cellular_component	UBC13-MMS2 complex
I	0031624	molecular_function	ubiquitin conjugating enzyme binding
I	0032991	cellular_component	protein-containing complex
I	0042275	biological_process	error-free postreplication DNA repair
I	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
I	0045893	biological_process	positive regulation of DNA-templated transcription
I	0061631	molecular_function	ubiquitin conjugating enzyme activity
I	0070062	cellular_component	extracellular exosome
I	0070534	biological_process	protein K63-linked ubiquitination
I	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
I	1902533	biological_process	positive regulation of intracellular signal transduction
K	0000151	cellular_component	ubiquitin ligase complex
K	0000166	molecular_function	nucleotide binding
K	0000209	biological_process	protein polyubiquitination
K	0000724	biological_process	double-strand break repair via homologous recombination
K	0000729	biological_process	DNA double-strand break processing
K	0003723	molecular_function	RNA binding
K	0004842	molecular_function	ubiquitin-protein transferase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006281	biological_process	DNA repair
K	0006282	biological_process	regulation of DNA repair
K	0006301	biological_process	DNA damage tolerance
K	0006511	biological_process	ubiquitin-dependent protein catabolic process
K	0006513	biological_process	protein monoubiquitination
K	0006974	biological_process	DNA damage response
K	0016567	biological_process	protein ubiquitination
K	0016740	molecular_function	transferase activity
K	0031371	cellular_component	ubiquitin conjugating enzyme complex
K	0031372	cellular_component	UBC13-MMS2 complex
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0032991	cellular_component	protein-containing complex
K	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
K	0043130	molecular_function	ubiquitin binding
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0045739	biological_process	positive regulation of DNA repair
K	0050852	biological_process	T cell receptor signaling pathway
K	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
K	0061631	molecular_function	ubiquitin conjugating enzyme activity
K	0070062	cellular_component	extracellular exosome
K	0070534	biological_process	protein K63-linked ubiquitination
K	0097027	molecular_function	ubiquitin-protein transferase activator activity
K	0140374	biological_process	antiviral innate immune response
K	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
K	1902533	biological_process	positive regulation of intracellular signal transduction
K	1904262	biological_process	negative regulation of TORC1 signaling
K	2000781	biological_process	positive regulation of double-strand break repair
L	0000151	cellular_component	ubiquitin ligase complex
L	0000209	biological_process	protein polyubiquitination
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006282	biological_process	regulation of DNA repair
L	0006301	biological_process	DNA damage tolerance
L	0006355	biological_process	regulation of DNA-templated transcription
L	0030154	biological_process	cell differentiation
L	0031371	cellular_component	ubiquitin conjugating enzyme complex
L	0031372	cellular_component	UBC13-MMS2 complex
L	0031624	molecular_function	ubiquitin conjugating enzyme binding
L	0032991	cellular_component	protein-containing complex
L	0042275	biological_process	error-free postreplication DNA repair
L	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
L	0045893	biological_process	positive regulation of DNA-templated transcription
L	0061631	molecular_function	ubiquitin conjugating enzyme activity
L	0070062	cellular_component	extracellular exosome
L	0070534	biological_process	protein K63-linked ubiquitination
L	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
L	1902533	biological_process	positive regulation of intracellular signal transduction
S	0004842	molecular_function	ubiquitin-protein transferase activity
S	0016567	biological_process	protein ubiquitination
T	0004842	molecular_function	ubiquitin-protein transferase activity
T	0016567	biological_process	protein ubiquitination
U	0004842	molecular_function	ubiquitin-protein transferase activity
U	0016567	biological_process	protein ubiquitination
V	0004842	molecular_function	ubiquitin-protein transferase activity
V	0016567	biological_process	protein ubiquitination

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNVdkl.GrICLdiL

Chain	Residue	Details
B	TYR80-LEU95

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)","evidences":[{"source":"PubMed","id":"16112642","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16122702","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	540
Details	Domain: {"description":"UBC core","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	148
Details	Domain: {"description":"U-box"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q9UNE7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"Q9UNE7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---