2B3Q

Crystal structure of a well-folded variant of green fluorescent protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	green fluorescent protein	polymer	246	27766.1	4	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
2	E, F, G (A)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
3	H, I, J, K (A, B, C, D)	water	water		18.0	455	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 238 (UniProt: P42212)

PDB	External Database	Details
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Gly 239	-	expression tag
Ser 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	111064.6
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	111137.5

*Water molecules are not included.