2B3Q

Crystal structure of a well-folded variant of green fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.07
Spacegroup name	P 21 21 21
Unit cell lengths	85.407, 87.160, 145.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.206
Rwork	0.206
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9c
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.900
High resolution limit [Å]	2.200
Number of reflections	44852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 3000, Ca(OAc)2, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K