2B3P

Crystal structure of a superfolder green fluorescent protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	green fluorescent protein	polymer	246	27772.2	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria
2	A	CADMIUM ION	non-polymer		112.4	9	Chemie (CD)
3	A	ACETIC ACID	non-polymer		60.1	6	Chemie (ACY)
4		water	water		18.0	328	Chemie (HOH)

Sequence modifications

A: 1 - 238 (UniProt: P42212)

PDB	External Database	Details
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation
Val 206	Ala 206	engineered mutation
Gly 239	-	expression tag
Ser 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27772.2
Non-Polymers*	Number of molecules	15
	Total formula weight	1372.0
All*	Total formula weight	29144.2

*Water molecules are not included.