2ASH

Crystal structure of Queuine tRNA-ribosyltransferase (EC 2.4.2.29) (tRNA-guanine (tm1561) from THERMOTOGA MARITIMA at 1.90 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Queuine tRNA-ribosyltransferase	polymer	381	42945.4	4	UniProt (Q9X1P7) Pfam (PF01702) In PDB	Thermotoga maritima	tRNA-guanine transglycosylase, Guanine insertion enzyme
2	A, B, C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A, C, D	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
4	A, B, C, D	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
5		water	water		18.0	655	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 369 (UniProt: Q9X1P7)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Ile -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	171781.6
Non-Polymers*	Number of molecules	19
	Total formula weight	1059.6
All*	Total formula weight	172841.2

*Water molecules are not included.