2ASH

Crystal structure of Queuine tRNA-ribosyltransferase (EC 2.4.2.29) (tRNA-guanine (tm1561) from THERMOTOGA MARITIMA at 1.90 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	169.467, 99.422, 124.163
Unit cell angles	90.00, 123.80, 90.00

Refinement procedure

Resolution	51.700 - 1.900
Rwork	0.193
R-free	0.22900
Structure solution method	MAD, MOLECULAR REPLACEMENT
Starting model (for MR)	1efz
RMSD bond length	0.017
RMSD bond angle	1.583
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0013)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.700	51.700	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.059	0.039	0.659
Number of reflections	133817
<I/σ(I)>	6.6	15.6	0.4
Completeness [%]	99.4
Redundancy	3.4	3.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		10	293	15.0% PEG-8000, 0.2M NaCl, 0.1M CHES pH 10.0 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K
1		10	293	15.0% PEG-8000, 0.2M NaCl, 0.1M CHES pH 10.0 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K