2AL2

Crystal Structure Analysis of Enolase Mg Subunit Complex at pH 8.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	enolase 1	polymer	436	46674.7	1	UniProt (P00924) Pfam (PF03952) Pfam (PF00113) In PDB	Saccharomyces cerevisiae (baker's yeast)	2-phosphoglycerate dehydratase; 2-phospho-D- glycerate hydro-lyase
2	B	enolase 1	polymer	436	46734.8	1	UniProt (P00924) Pfam (PF03952) Pfam (PF00113) In PDB	Saccharomyces cerevisiae (baker's yeast)	2-phosphoglycerate dehydratase; 2-phospho-D- glycerate hydro-lyase
3	A, B	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	A, B	PHOSPHOENOLPYRUVATE	non-polymer		168.0	2	Chemie (PEP)
5	A, B	2-PHOSPHOGLYCERIC ACID	non-polymer		186.1	2	Chemie (2PG)
6	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	B	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
8		water	water		18.0	535	Chemie (HOH)

Sequence modifications

A: 1 - 436 (UniProt: P00924)

PDB	External Database	Details
Ala 345	Lys 345	engineered mutation

B: 1 - 436 (UniProt: P00924)

PDB	External Database	Details
Asp 80	Asn 80	engineered mutation
Asp 126	Asn 126	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93409.5
Non-Polymers*	Number of molecules	10
	Total formula weight	880.0
All*	Total formula weight	94289.4

*Water molecules are not included.