2AL2
Crystal Structure Analysis of Enolase Mg Subunit Complex at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-04-04 |
| Detector | BRUKER |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.700, 65.600, 85.900 |
| Unit cell angles | 90.00, 99.20, 90.00 |
Refinement procedure
| Resolution | 26.000 - 1.850 |
| R-factor | 0.1961 |
| Rwork | 0.196 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.240 |
| Data reduction software | SADABS |
| Data scaling software | SAINT |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 67650 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 8 | 293 | PEG 8000, potasssium chloride, pH 8.0, Batch, temperature 293K |
