2ABZ

Crystal structure of C19A/C43A mutant of leech carboxypeptidase inhibitor in complex with bovine carboxypeptidase A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Carboxypeptidase A1	polymer	309	34721.8	2	UniProt (P00730) Pfam (PF00246) In PDB	Bos taurus (cattle)	carboxypeptidase A, CPA, A/B metallocarboxypeptidase
2	C, D, E, F	Metallocarboxypeptidase inhibitor	polymer	67	7333.1	4	UniProt (P81511) In PDB	Hirudo medicinalis (medicinal leech)	Leech carboxypeptidase inhibitor, LCI, inhibitor of A/B metallocarboxypeptidases
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 1 - 309 (UniProt: P00730)

PDB	External Database	Details
Ala 228	Glu 338	SEE REMARK 999
Val 305	Leu 415	SEE REMARK 999

C, D, E, F: 2 - 67 (UniProt: P81511)

PDB	External Database	Details
Gly 1	-	cloning artifact
Ala 19	Cys 33	engineered mutation
Ala 43	Cys 57	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	98776.0
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	98906.8

*Water molecules are not included.