29TE
The complex of A1AT-NHK with the ERAD misfolded glycoprotein checkpoint complex from Chaetomium thermophilum (EDEM:PDI heterodimer).
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green fluorescent protein,alpha-1,2-Mannosidase | polymer | 1284 | 142162.8 | 1 | UniProt (P42212) UniProt (G0SCX7) | Aequorea victoria (Water jellyfish Mesonema victoria) | |
| 2 | B (B) | Protein disulfide-isomerase | polymer | 507 | 55936.4 | 1 | UniProt (G0SGS2) | Thermochaetoides thermophila | |
| 3 | C, G, I (C, G, I) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 3 | In PDB GlyTouCan (G81315DD) | |||
| 4 | D, F (D, F) | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose | branched | 342.3 | 2 | In PDB GlyTouCan (G77384RA) | |||
| 5 | E, H (E, H) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 6 | J, L, M, R, S (A, B) | alpha-D-mannopyranose | non-polymer | 180.2 | 5 | Chemie (MAN) PubChem (185698) | |||
| 7 | K (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) PubChem (271) | |||
| 8 | N (A) | KIFUNENSINE | non-polymer | 232.2 | 1 | Chemie (KIF) PubChem (130611) | |||
| 9 | O, P (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) PubChem (24139) | |||
| 10 | Q (A) | beta-D-mannopyranose | non-polymer | 180.2 | 1 | Chemie (BMA) PubChem (439680) |
Sequence modifications
A: -177 - 55 (UniProt: P42212)
A: 63 - 1092 (UniProt: G0SCX7)
B: 21 - 517 (UniProt: G0SGS2)
| PDB | External Database | Details |
|---|---|---|
| Met -182 | - | initiating methionine |
| Val -181 | - | expression tag |
| Ser -180 | - | expression tag |
| Lys -179 | - | expression tag |
| Gly -178 | - | expression tag |
| Leu -119 | Phe 64 | engineered mutation |
| Thr -118 | Ser 65 | engineered mutation |
| Leu 48 | His 231 | engineered mutation |
| Leu 56 | - | linker |
| Glu 57 | - | linker |
| Val 58 | - | linker |
| Leu 59 | - | linker |
| Phe 60 | - | linker |
| Gln 61 | - | linker |
| Gly 62 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 1093 | - | expression tag |
| Thr 1094 | - | expression tag |
| Lys 1095 | - | expression tag |
| His 1096 | - | expression tag |
| His 1097 | - | expression tag |
| His 1098 | - | expression tag |
| His 1099 | - | expression tag |
| His 1100 | - | expression tag |
| His 1101 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 18 | - | expression tag |
| Thr 19 | - | expression tag |
| Gly 20 | - | expression tag |
| Lys 518 | - | expression tag |
| His 519 | - | expression tag |
| His 520 | - | expression tag |
| His 521 | - | expression tag |
| His 522 | - | expression tag |
| His 523 | - | expression tag |
| His 524 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 198099.3 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 4103.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1795.6 | |
| All* | Total formula weight | 203998.6 |
