29AJ

Crystal Structure of the human mARC1 M187K variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Mitochondrial amidoxime-reducing component 1,Endolysin	polymer	455	51410.9	1	UniProt (Q5VT66) UniProt (P00720) Pfam (PF03476) Pfam (PF00959) Pfam (PF03473)	Homo sapiens (human)	mARC1,Molybdenum cofactor sulfurase C-terminal domain-containing protein 1,MOSC domain-containing protein 1,Moco sulfurase C-terminal domain-containing protein 1,Lysis protein,Lysozyme,Muramidase
2	B (D)	PHOSPHONIC ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER	non-polymer		395.4	1	Chemie (MTE)
3	C (D)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	1	Chemie (B3P) PubChem (125132)
4	D, E, F, G (D)	MOLYBDATE ION	non-polymer		159.9	4	Chemie (MOO) PubChem (24621)
5	H (D)	oxidanyl(oxidanylidene)molybdenum	non-polymer		128.9	1	Chemie (EFK) PubChem (6386441)
6	I (D)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
7	J (D)	water	water		18.0	584	Chemie (HOH)

Sequence modifications

D: 53 - 128 (UniProt: Q5VT66)

PDB	External Database	Details
Met 52	-	initiating methionine

D: 129 - 287 (UniProt: P00720)

PDB	External Database	Details
Gly 140	Arg 12	conflict
Arg 265	Ile 137	conflict

D: 288 - 496 (UniProt: Q5VT66)

PDB	External Database	Details
Ala 324	Thr 165	conflict
Lys 346	Met 187	engineered mutation
Gly 497	-	expression tag
Thr 498	-	expression tag
Val 499	-	expression tag
Asp 500	-	expression tag
His 501	-	expression tag
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51410.9
Non-Polymers*	Number of molecules	8
	Total formula weight	1481.8
All*	Total formula weight	52892.7

*Water molecules are not included.